Base Broth Ingredients: Peptone (2.5g) Beef Extract (0.25g) Sodium Chloride (1.25g) Phenol Red (2mL) Distilled Water (250mL) a) What is the purpose

Base Broth Ingredients:

  1. Peptone (2.5g)
  2. Beef Extract (0.25g)
  3. Sodium Chloride (1.25g)
  4. Phenol Red (2mL)
  5. Distilled Water (250mL)

a) What is the purpose of each ingredient in broth mixture?

b) If phenol red was not available for preparing the medium, what suitable pH indicator(s) may be used? How would this impact the test?

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