Can you describe this process?

Can you describe this process?

To move the shRNA expression units from pSilencer

into pUMVC3 vector, PCR primer was used to change

the BamHI sites to SalI site (BamHI to SalI primer, 50-AAGTCGACTGCTGTTGACAGTG-30),

PCR amplification with F seq primer (50-AGGCGATTAAGTTGGGTA-30), and the resulting PCR fragments were digested

with SalI and NotI and inserted into the SalI and NotI sites of pUMVC3.   

Place New Order
It's Free, Fast & Safe

"Looking for a Similar Assignment? Order now and Get a Discount!